Council Officer – Young Investigator Group
Vascular Cell Biology Lab| Sanquin Research and Landsteiner Laboratory
Leeuwenhoek Centre for Advanced Microscopy (LCAM)
Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam
Jaap D. van Buul is professor of Vascular Cell Biology by special appointment at the Swammerdam Institute for Life Sciences (SILS) at University of Amsterdam. He did his postdoc in the lab of Prof. Dr. Keith Burridge at the department of Developmental and Cell Biology at the University of North Carolina in Chapel Hill, USA. This was a wonderful period and showed for the first time a functional role for the small GTPase RhoG. In 2009, he co-founded the Dutch Endothelial Biology Society (DEBS), a society that gives a platform to young investigators in the vascular biology field to present their work to a larger audience and currently holds the chair. In addition, Prof. Van Buul is the president of the Dutch Society for Cell Biology (DSCB), formally known as the NVvC. In 2016, he joined the board of the European Vascular Biology Organization (EVBO) and in 2018 became a member of the F1000. Currently, Prof. Van Buul runs the Vascular Cell Biology lab at Sanquin Research in Amsterdam.
The Van Buul lab uses several cellular and molecular biology techniques to visualize the actual adhesion and transmigration of immune cells across the endothelium. The lab uses transmigration assay under physiological flow in combination with advanced confocal laser scanning microscopy and in collaboration lattice light sheet microscopy. This allows following transendothelial migration with the highest possible resolution in 3D in time with minimal phototoxicity, enabling us to draw conclusions on the spatial and temporal regulation of all different steps of the transendothelial migration cascade. Most recently, the lab entered the field of optogenetics using FRET-based biosensors: a technique that reveals the localization of protein activation through the transmission of fluorescent signals. Recently, the lab started generating vessel-on-a-chip. With the use of all described tools, it is the goal to understand the molecular details of leukocyte transendothelial migration to ultimately develop therapies that either promote or inhibit leukocyte transendothelial migration and vascular permeability in an organ-specific manner.